Journal: Journal of visualized experiments : JoVE

Article Title: Cell-free Protein Expression Using the Rapidly Growing Bacterium Vibrio natriegens

doi: 10.3791/59495

Figure Lengend Snippet: Table of Materials

Article Snippet: As we unravel the biology underlying its rapid growth, further development of V. natriegens cell- free systems may accelerate bioproduction capabilities and enable robust expression of therapeutic peptides, small molecules, and synthetic materials. table ft1 table-wrap mode="anchored" t5 Name Company Catalog Number Comments 15 mL Tubes Corning 352196 2 mL Tubes Eppendorf 22363352 384-well Black Assay Plates Corning 3544 384-well PCR Plates Eppendorf 951020702 50 mL Tubes Corning 352070 96-well PCR Plates Eppendorf 30129300 Adenosine 3',5'-cyclic monophosphate sodium salt monohydrate Sigma A6885 Adenosine 5'-triphosphate - 100 mM NEB N0450L Applied Biosystems Veriti 384-well Thermocycler ThermoFisher 4388444 Assay Plate Adhesives BioRad MSB1001 β-Nicotinamide adenine dinucleotide hydrate (NAD) Sigma/Roche 10127965001 Coenzyme A hydrate Sigma C4283 Cytidine 5'-triphosphate - 100 mM NEB N0450L D-(-)-3-Phosphoglyceric acid disodium salt (3-PGA) Sigma P8877 Dewar Flask - 4L ThermoScientific 10-194-100C DL-Dithiothreitol solution - 1 M Sigma 42816 Folinic acid calcium salt hydrate Sigma 47612 Glacial Acetic Acid Sigma A6283 Guanosine 5'-triphosphate - 100 mM NEB N0450L HEPES Sigma H3375 L-Glutamic acid hemimagnesium salt tetrahydrate Sigma 49605 L-Glutamic acid potassium salt monohydrate Sigma G1149 LB Broth (Miller) Sigma L3522 Magnesium chloride (MgCl2) Sigma M8266 Plasmid pJL1-sfGFP Addgene 69496 Plasmid Plus Maxi kit Qiagen 12963 Poly(ethylene glycol) (PEG)-8000 Sigma 89510 Potassium chloride (KCl) Sigma P9333 Potassium hydroxide Pellets Sigma/Roche 1050121000 Q125 Sonicator and CL-18 probe with a?-inch tip Qsonica 4422 RNA Clean and Concentrator Kit Zymo R1013 RNase Inhibitor, Murine NEB M0314 RTS Amino Acid Sampler Biotechrabbit BR1401801 Sodium chloride (NaCl) Sigma S7653 Spermidine Sigma S0266 T7 RNA Polymerase NEB M0251 Tris Solution (pH 8.0) - 1 M Invitrogen AM9856 tRNA from E. coli MRE 600 Sigma/Roche 10109541001 Uridine 5'-triphosphate - 100 mM NEB N0450L Vibrio natriegens (Wild-type) Lyophilized Stock ATCC 14048 Open in a separate window

Techniques: Plasmid Preparation